Sanger sequencing- Part 3 In 1977, Fredrick. Sanger introduced chain elongation termination method, in which elongated chain is terminated by special nucleotides. Sanger sequencing is much better and safer than Maxam and Gilbert method because there is no use of…
Maxam-Gilbert Chemical-Degradation Sequencing– Part 2 Today we will discuss about Maxam-Gilbert Chemical-Degradation Sequencing. In my last blog we learn about the Ray Wu’s Primer-Extension Approach, but due to its own limitation Maxam-Gillbert chemical degradation sequencing method comes into the picture.…
Ray Wu’s Primer-Extension Method– Part 1 DNA sequencing is process that are used to determine the exact sequence of nucleotides in the DNA. There are different techniques which are used for Nucleotides ((adenine – A, thymine – T, cytosine –…
#1 How to isolate plasmid and improve its quality The method’s basic idea is to selectively denature high molecular weight chromosomal DNA using alkaline denaturation, leaving covalently closed circular DNA double-stranded. After neutralization, plasmid DNA remains in the supernatant, and…
